《国际小儿耳鼻咽喉头颈外科杂志》最新一项针对多种增强鼓膜（TM）穿孔愈合辅助治疗疗效的研究指出，尽管许多研究展示了有前景的结果，但是仍然有许多问题亟待解决。

　　来自加拿大的研究者针对TM穿孔检索了PubMed2000年1月至2012年6月之间的文献，包括引文。纳入和排除的标准适用于所有评估辅助疗法对TM愈合的实验研究。

　　结果发现，许多研究已经评估了生物分子或者生长因子，比如表皮生长因子和成纤维细胞生长因子的疗效，在TM再生方面有重大的成功。TM组织工程最近的策略涉及生物工程支架材料，比如丝纤蛋白，壳聚糖和脱细胞的细胞外基质。大多数支架材料展示了良好的生物相容性和更快的TM穿孔愈合速率。

　　研究者指出，尽管许多研究展示了有前景的结果，但是仍然有许多问题亟待解决，比如动物模型的充分性和辅助材料的长期生物相容性。同时，在临床应用之前需进一步对众多辅助成分和生物支架进行对比研究。

　　Repair of tympanic membrane perforation using novel adjuvant therapies: A contemporary review of experimental and tissue engineering studies.

　　Hong P, Bance M, Gratzer PF.

　　SourceIWK Health Centre, Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, Dalhousie University, Halifax, Nova Scotia, Canada; School of Human Communication Disorders, Dalhousie University, Halifax, Nova Scotia, Canada.

　　Abstract

　　OBJECTIVE:To perform a contemporary review of experimental studies to describe the effects of various novel adjuvant therapies in enhancing tympanic membrane (TM) perforation healing.

　METHODS:A PubMed search for articles from January 2000 to June 2012 related to TM perforation, along with the references of those articles, was performed. Inclusion and exclusion criteria were applied to all experimental studies assessing adjuvant therapies to TM healing.

RESULTS:Many studies have assessed the efficacy of biomolecules or growth factors, such as epidermal growth factors and basic fibroblast growth factors, in TM regeneration with significant success. More recent strategies in TM tissue engineering have involved utilizing bioengineered scaffold materials, such as silk fibroin, chitosan, calcium alginate, and decellularized extracellular matrices. Most scaffold materials demonstrated biocompatibility and faster TM perforation healing rates.

CONCLUSION:Although several studies have demonstrated promising results, many questions still remain, such as the adequacy of animal models and long-term biocompatibility of adjuvant materials. As well, further studies comparing various adjuvant substances and bioscaffolds are required prior to clinical application.